The invention discloses a GU-25KF type manual high vacuum ball valve. The GU-25KF type manual high vacuum ball valve comprises two quick-disassembling flanges, the two quick-disassembling flanges areseparately arranged on the left and right end faces of a valve body part through two second bolts, a name plate is arranged on the front face of the valve body part, and first gaskets are connected tothe outer surfaces of the second bolts in a sleeved mode. First seal rings are arranged between the left and right side faces of the valve body part and the two quick-disassembling flanges, and sealing seats are arranged at four corners of an inner cavity of the valve body part. According to the GU-25KF type manual high vacuum ball valve, by arranging a fourth bolt, a valve rod and a handle partare connected through the fourth bolt and an elastic cylindrical pin, so that a ball body, the valve rod and the handle part can form a main driving structure of the valve, the leak-proofness of the high vacuum ball valve is improved, the working stability of the high vacuum ball valve is enhanced, the service lift of the high vacuum ball valve is guaranteed, and the replacement times of the highvacuum ball valve are reduced.