The invention provides a method for applying an acquisition and operation and maintenance knowledge base in an on-site work terminal. The method comprises the following steps: the on-site work terminal is logged in and a remote acquisition and operation and maintenance knowledge base is downloaded to the on-site work terminal to generate an on-site acquisition and operation and maintenance knowledge base; the on-site work terminal receives a work order issued by the remote acquisition and operation and maintenance knowledge base, wherein the work order is an integrated execution script, the execution script being formed by a plurality of atomic operation in sequence, the atomic operation being indivisible and executable operation; the on-site work terminal analyzes the execution script inthe work order to obtain the atomic operation included in the execution script and execution sequence thereof; and the on-site work terminal calls the atomic operation obtained after analysis in the on-site acquisition and operation and maintenance knowledge base in sequence according to the execution sequence, and automatically records execution result of each atomic operation. The method realizes fast determination and solution of fault by applying the acquisition and operation and maintenance knowledge base to the on-site work terminal to assist field operation.