The invention belongs to the field of pesticides, and particularly relates to a nematicidal preparation containing abamectin and T.longibrachiatum. The preparation is prepared from an abamectin solution and T.longibrachiatum bacteria liquid, so that an effect of toxically killing wheat cereal cyst nematode can be remarkably improved, a slow release effect is achieved, the lasting period of a pesticide can be prolonged, and the preparation is non-pollution and good in environmental compatibility, achieves low production cost, is capable of preventing nematode from easily becoming resistant to drugs, and has the effects of enlarging a prevention and cure spectrum and improving synergistic interaction, and the like.