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发明公开
CN108374016A 一种黄姑鱼超氧化物歧化酶MnSOD基因及其应用 无效 - 驳回

一种黄姑鱼超氧化物歧化酶MnSOD基因及其应用
摘要：
本发明公开了一种黄姑鱼超氧化物歧化酶MnSOD基因及其应用，其cDNA包括如SEQ ID NO 01所示的序列。本发明首次从黄姑鱼的肝脏组织中克隆到一种超氧化物歧化酶MnSOD基因的cDNA。采用本发明试剂盒首次研究氨氮急性攻毒后，黄姑鱼超氧化物歧化酶MnSOD基因mRNA表达的时相变化，该mRNA表达水平的检测在氨氮对黄姑鱼的污染检测中作为一种生物标记，在黄姑鱼对氨氮环境预警中有重要的应用前景，并为黄姑鱼的健康状态和生长环境安全的检测奠定基础。
摘要（英）：

The invention discloses a Nibea albiflora manganese superoxide dismutase MnSOD gene and application thereof. A cDNA (complementary deoxyribonucleic acid) of the gene comprises a sequence as showed inSEQ ID NO. 01. According to the gene, the cDNA of the MnSOD gene is firstly cloned from liver tissues of Nibea albiflora. By the aid of a kit, phase change is firstly researched when mRNA (messenger ribonucleic acid) of the Nibea albiflora MnSOD gene is expressed after acute challenge of ammonia nitrogen is researched for the first time, detection of the expression level of the mRNA serves as a biomarker in ammonia nitrogen pollution detection of the Nibea albiflora, the gene has an important application prospect in ammonia nitrogen environment warning of the Nibea albiflora, and a foundationis laid for the detection of health states and growth environment safety of the Nibea albiflora.

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