The invention discloses a final segment saturation control method of non-cooperative target spacecraft rendezvous and docking. The method comprises: a spatial coordinate system including an earth inertial coordinate system, an orbital coordinate system, a target spacecraft body coordinate system, a tracking spacecraft coordinate system and a line-of-sight coordinate system is defined and a kinematical equation of the line-of-sight coordinate system is established; a six-degree-of-freedom rendezvous and docking model for the spacecraft is established; a two-stage artificial potential function is established; and according to bounded gradient information of the two-stage artificial potential function, a six-degree-of-freedom saturation controller for spacecraft rendezvous and docking is designed. According to the invention, a novel line-of-sight coordinate system is designed and thus a problem of real-time measurement of the sensitive device of the spacecraft system is solved; with introduction of the saturation controller based on the two-stage artificial potential function, a safety rendezvous problem in spacecraft rendezvous and docking and problems of limited control input saturation and external disturbance influence are solved; and the system robustness is ensured.