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发明公开
CN108387735A 一种基于双分子荧光互补技术的AFP检测试剂盒、制备及使用方法 无效 - 撤回

一种基于双分子荧光互补技术的AFP检测试剂盒、制备及使用方法
摘要：
本发明提供了一种基于双分子荧光互补技术的AFP诊断试剂盒。所述试剂盒包括：抗AFP抗体偶联的荧光蛋白N端片段、抗AFP抗体偶联的荧光蛋白C端片段；本发明还公开了所述一种基于双分子荧光互补技术的AFP诊断试剂盒的制备方法，该方法包括：抗AFP抗体偶联的荧光蛋白N端片段的制备、抗AFP抗体偶联的荧光蛋白C端片段的制备；最后还公开了该试剂盒的使用方法；本发明试剂盒具有操作简单、免清洗、精密度好、准确度高等优点，便于临床检测使用，其应用于原发性肝癌的监测，可以提高原发性肝癌诊断的准确率，具有极大的市场价值。
摘要（英）：

The invention provides an AFP diagnosis kit based on a bimolecular fluorescence complementation technology, wherein the kit comprises an anti-AFP antibody conjugated fluorescent protein N-terminal fragment and an anti-AFP antibody conjugated fluorescent protein C-terminal fragment. The invention further discloses a preparation method of the AFP diagnosis kit, wherein the preparation method comprises: preparation of an anti-AFP antibody conjugated fluorescent protein N-terminal fragment, and preparation of an anti-AFP antibody conjugated fluorescent protein C-terminal fragment. The invention further discloses a use method the kit. According to the present invention, the kit has advantages of simple operation, no cleaning, high precision, high accuracy and the like, is conveniently suitablefor clinical detection, can increase the accuracy of the diagnosis of primary liver cancer in the monitoring of primary liver cancer, and has great market value.

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