The invention discloses a universal scanning electron microscope sample stage base. The universal scanning electron microscope sample stage base comprises a top surface for loading sample stages, andat least one support column, wherein the top surface is provided with at least one sample stage hole; each sample stage hole is used for holding a cylindrical sample stage; and the support column hasan external thread matched with a screw hole of a screw-hole-type sample stage. The sample stage base can match most models of scanning electron microscope sample stages in the market, so that observation efficiency of the sample stage base is greatly improved, and observation cost of a scanning electron microscope is obviously reduced. The universal scanning electron microscope sample stage baseis compact in layout, and the limited space of the sample stage base is fully and reasonably utilized.