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发明授权
CN108409805B 一种飞燕草素-3-O-半乳糖苷的分离纯化方法及其应用 有权

一种飞燕草素-3-O-半乳糖苷的分离纯化方法及其应用
摘要：
本发明公开了一种飞燕草素‑3‑O‑半乳糖苷的分离纯化方法及其在制备α‑葡萄糖苷酶抑制剂中的应用，该分离纯化方法以花色苷组成复杂的蓝莓为原料，包括醇提浓缩、大孔树脂吸附、制备液相色谱纯化和高速逆流色谱分离，通过将制备液相色谱和高速逆流色谱技术结合，再通过对工艺参数的优化，分离制备得到高纯度的飞燕草素‑3‑O‑半乳糖苷单体，纯度高达99％；经活性试验发现，该飞燕草素‑3‑O‑半乳糖苷单体能够显著抑制α‑葡萄糖苷酶的活性，可用于制备α‑葡萄糖苷酶抑制剂。
摘要（英）：

The invention discloses a delphinidin-3-O-galactoside separation and purification method and an application thereof to preparation of an alpha-glucosidase inhibitor. The separation and purification method takes blueberries with complex anthocyanin compositions as raw materials and includes alcohol extraction concentration, macroporous resin adsorption, preparative liquid phase chromatography purification and high-speed counter-current chromatography separation, preparative liquid phase chromatography and high-speed counter-current chromatography technologies are combined, process parameters are optimized, a high-purity delphinidin-3-O-galactoside monomer is prepared by separation, and the purity of the monomer reaches up to 99%. Activity tests discover that the delphinidin-3-O-galactosidemonomer can remarkably inhibit activity of alpha-glucosidase and can be used for preparing the alpha-glucosidase inhibitor.

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