The invention discloses a device and a method for evaluating the carbon dioxide huffing-puffing recovery rate of shale oil. The device is characterized in that a radial rock core holder, a confining pressure pump and a first pressure sensor are respectively connected with a first tee joint, a simulated oil saturation pump, a third pressure sensor and the radial rock core holder are respectively connected with a fourth tee joint, the rock core holder, an oil-gas separator and a vacuum pump are respectively connected with a third tee joint, the oil-gas separator is communicated with the outside,the rock core holder is connected with a visual container, and the visual container, a second pressure sensor and a micro-injection pump are connected through a second tee joint; a CO2 storage tank is connected with the visual container; and the whole device controls the temperature through a thermotank. The device and the method allow the micro-crack and the matrix recovery rate to be respectively obtained, can analysis the contribution of adsorbed dissolved crude oil in organic matters to the recovery rate, and are of great significance to design an onsite exploitation scheme.