The invention discloses a one-key sequential control method for an intelligent transformer substation protection device, proposes the sequential control for the protection device, achieves the one-keysequential control of switching protection and safety isolation, and effectively reduces the risk of misoperation in the safety measure process through the fixing of the switching step of the protection device. The above technical scheme of the invention is implemented at one 220kV intelligent transformer substation, and the operation state is better, thereby greatly improving the intelligentization level of the transformer substation.