The invention discloses an acquisition method of a standard image in automated optical inspection, and automated optical inspection equipment. The method comprises the following steps: continuously acquiring a scanned image of a to-be-inspected object; acquiring the difference between the scanned image and the current standard image; determining whether to update the current standard image according to the difference; and updating the current standard image by using the scanned image if needing to update the current standard image, thereby obtaining the final target standard image. Through themethod disclosed by the invention, the situation that the obtained target standard image is free from defect can be guaranteed, and then a detection effect of performing defect detection by using thetarget standard image can be improved.