The invention belongs to the technical field of disinfectants, and particularly relates to a povidone-iodine solution and a preparation method thereof. The povidone-iodine solution provided by the invention is prepared from povidone-iodine, monopotassium phosphate, dipotassium phosphate, potassium iodate, glycerinum, aloe zinc-polysaccharide and sodium citrate. The povidone-iodine solution prepared by the preparation method has the advantages of high stability, remarkable sterilizing effect and durable effect. Meanwhile, the povidone-iodine solution provided by the invention also has the effects of stopping bleeding, clearing free radicals in wounds and preventing wound pollution, and can effectively facilitate wound healing. When the povidone-iodine solution is applied to bathing of piglets, the disease infection prevention ability of piglets can be enhanced, and adverse reactions due to stress reaction can be improved, so that the health level of the piglets is raised. The povidone-iodine solution is an ideal disinfection liquid suitable for piglets.