The invention relates to a method for detecting astragaloside content by high performance liquid chromatograph. The method comprises the following steps: preparing an astragaloside sample to be detected; drying; crushing; adding an extracting solvent, wherein the extracting solvent comprises methyl alcohol, 65-75% ethyl alcohol and ammonia water based on the volume ratio of (8-10): (0.1-0.5): (0.5-1.5); fully extracting; filtering; collecting filtrate; and evaporating and drying; and then dissolving. According to the method, the research of the extracting solvent is optimized, so that astragaloside can be separated from other similar impurities to the maximum and the astragaloside can be fully extracted on the premise that the extracting solvent is not wasted and the purifying step is simplified, and as a result, the foundation is provided to realize accurate detection; furthermore, the detection conditions of the liquid chromatography are further optimized; a special flowing phase anda special detecting device are selected, so that the detecting accuracy can be effectively improved; and the method has the advantages of being high in specificity, and high in sensitivity.