The invention relates to a silica and zinc oxide anti-reflection film for dye-sensitized solar cells and a preparation method thereof. SiO2 with a refractive index of 1.3 is placed on the glass surface of the conductive glass FTO, and ZnO with a refractive index of 1.9 is placed on the conductive surface of the conductive glass FTO, which forms a gradient change with the refractive index of the conductive glass to improve the transmission of the conductive glass, thereby finally improving the efficiency of dye-sensitized solar cells. The anti-reflection film obtained by the invention can not only improve the transmittance of the conductive glass, but also enhance the electron transport efficiency, and is used for the quasi-solid-state dye-sensitized solar cell, and the photoelectric conversion efficiency of the cell can be increased from 4.71% to 5.63. %.