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发明公开
CN108498863A 一种复合口腔粘膜上皮细胞的口腔补片制备方法 无效 - 撤回

一种复合口腔粘膜上皮细胞的口腔补片制备方法
摘要：
本发明公开一种复合口腔粘膜上皮细胞的口腔补片制备方法，包括以下步骤：采用粘膜下层制备口腔补片，通过传代培养法培养口腔粘膜上皮细胞，将口腔粘膜上皮细胞接种至放有口腔补片的细胞培养皿中，37℃，5%CO2细胞培养箱中培养，培养终止后即得到复合口腔粘膜上皮细胞的口腔补片。本发明所提供的一种复合口腔粘膜上皮细胞的口腔补片制备方法，材料来源充分，能够使口腔粘膜上皮细胞在口腔补片中大量增殖，为临床上口腔粘膜大面积缺损的修复重建提供新的途径。
摘要（英）：

The invention discloses an oral patch preparing method of composite oral mucosa epithelial cells, which includes the following steps: preparing the oral patch by using submucosa; culturing the oral mucosa epithelial cells by adopting a subculture method; inoculating the oral mucosa epithelial cells into a cell culture plate with the oral patch; culturing in a cell incubator with the temperature of37 DEG C and 5% CO2, and the oral patch of the composite oral mucosa epithelial cells is obtained once the culture is finished. The oral patch preparing method of the composite oral mucosa epithelialcells has the advantage that material sources are adequate, the oral mucosa epithelial cells can largely proliferate in the oral patch, and a new way is provided for clinic reconstruction oral mucosalarge area defects.

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