The invention discloses a traditional Chinese medicine preparation for degrading Zearalenone and a preparation method and application thereof, and belongs to the field of biological pollution and control. The traditional Chinese medicine preparation of the present invention is prepared by microwave extraction of crushed Chinese herbal components. The Chinese herbal components comprise (by weight):10-25 parts of dahurian angelica root, 10-20 parts of pekoe, 10-20 parts of Solanum dulcamara, 5-15 parts of climbing nightshade, 5-15 parts of radical lobelia, 5-10 parts of alum, 5-10 parts of whitepeony root, 3-5 pars of barbat skullcap, 1-3 parts of Wedelia wallichii Less and 5-15 parts of cynanchi root. The prepared traditional Chinese medicine preparation can effectively degrade Zearalenonewith irreversible reactions. In addition, the degradation rate of the traditional Chinese medicine extract for degrading 25-100 ng of Zearalenone is 100%, and the degradation rate of the traditionalChinese medicine extract for degrading 200-500 ng of Zearalenone is 100% is 98% and above. The traditional Chinese medicine extract can effectively degrade Zearalenone.