The invention discloses an anti-piracy CO2 safety release system and an operation method thereof. The anti-piracy CO2 safety release system comprises a release box, a release pipe and a safety pipe; the release box is arranged outside an engine room; a release pipe first end and a release pipe second end are arranged on the release pipe, wherein the release pipe first end is connected with the release box, and the release pipe second end extends into the engine room; and a safety air inlet and a safety air exhaust port are formed in the safety pipe, wherein the safety air inlet is selectivelyconnected with the release pipe second end, and the safety air exhaust port extends out of a top deck of a ship. By arranging the safety pipe, the release pipe second end is in communication with thesafety pipe when the ship is attacked by pirates; even though the pirates operate the release box to release CO2, the safety pipe can also guide the CO2 in the release pipe into the atmosphere outsidethe deck in time to guarantee that the CO2 is not inflated into the whole engine room; and when the ship is in normal sailing, the release pipe second end is in communication with the interior of theengine room to guarantee that the CO2 can be inflated into the engine room in time if needed to enhance the safety of the engine room.