The invention discloses an oedaleus asiaticus protein tyrosine kinase PTK as well as an encoding gene and application thereof. At first, the oedaleus asiaticus protein tyrosine kinase PTK is cloned from oedaleus asiaticus, a primer is designed for the oedaleus asiaticus protein tyrosine kinase PTK, and dsRNA used for interfering with the oedaleus asiaticus protein tyrosine kinase PTK is synthesized, and is introduced in the oedaleus asiaticus by an injection method to perform RNAi on the oedaleus asiaticus protein tyrosine kinase PTK. The result shows that after the dsRNA is injected into theoedaleus asiaticus, the survival rate, the growth rate, the weight and the overall expressiveness are remarkably reduced, and the oedaleus asiaticus protein tyrosine kinase PTK plays an important rolein the insect growth and development process. A new method is provided for regulation and control of injurious insect molecules, and a theoretical basis is provided for deeply understanding an insectgrowth and development mechanism and creating a new biopesticide preparation.