The invention relates to a breeding method based on a running water fish-breeding tank. A running water fish-breeding tank is established. Fish is bred in the established running water fish-breeding tank. The running water fish-breeding tank comprises a substrate keel rack (1), multiple groove-wall keel racks extending upwards from the partial top of the substrate keel rack, and water separation boards (4) covering the top of a remaining part of the substrate keel rack (1), tops and side surfaces of the groove-wall keel racks (3). Water separation boards (4) on the side surfaces of the groove-wall keel racks (3) and water separation boards (4) at the top of the remaining part of the substrate keel rack (1) form multiple fish-breeding water tanks (5). Aisles (6) are formed by the water separation boards (4) at tops of the multiple groove-wall keel racks (3). Oxygen-increasing devices (7) are arranged in the fish-breeding water tanks (5). The substrate keel rack (1) is equipped with a fixing pier (2) which extends downwards and is used for inserted underground. Through the breeding method, a fish-breeding coefficient is high so that fish can grow healthy.