The invention discloses a device for conducting electrostatic collection on sample particles by utilizing an electric charge applying method. The device is composed of an electron emission and acceleration part, and a particle collecting part; and the electron emission and acceleration part enables the sample particles to be charged through electric charge applying, and the particle collecting part has the function of collecting the sample particles. According to the device, the to-be-collected sample particles are charged negatively, and thus collection is completed under the action of an electrostatic field; and the device is simple in structure, small in size and high in collection efficiency, and the collection effect has nothing to do with the conductivity of the sample particles.