The invention relates to a culture medium for induced differentiation of pluripotent stem cells into hematopoietic stem cells and a method thereof. The culture medium comprises a first-stage culture medium and a second-stage culture medium. According to the method, the first-stage culture medium and the second-stage culture medium are successively used for two-stage co-culture of pluripotent stemcells and OP9 cells. In comparison with the prior art, the invention has the following beneficial effects: by the use of the culture medium with the above two specific ingredients and concentrations and for induced differentiation of pluripotent stem cells into hematopoietic stem cells, induced differentiation of pluripotent stem cells is successively conducted, and the efficiency of differentiation of pluripotent stem cells into hematopoietic stem cells is raised.