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Invention Grant
CN108588056B 一种低温α-淀粉酶Tcamy及其基因和应用 有权 转让

一种低温α-淀粉酶Tcamy及其基因和应用
Abstract:
本发明涉及农业生物技术领域，具体涉及一种来源于真菌的低温α‑淀粉酶Tcamy及其基因和应用。其氨基酸序列如SEQ ID NO.1或2所示。该淀粉酶Tcamy最适pH为5.0，在pH5.0‑pH10.0范围内，该酶能够维持其50％以上的酶活力，这说明此酶具有很好的pH稳定性。其最适温度35℃，在0℃下保持有27％的活性；当热处理温度超过其最适温度之后，酶活骤降,为典型的低温α‑淀粉酶。
Abstract(English):

The invention relates to the field of agricultural biotechnology, in particular to fungi rooted low temperature alpha-amylase Tcamy and a gene and an application thereof. The amino acid sequence is shown as SEQ ID NO.1 or 2. The optimum pH value for the amylase Tcamy is 5.0, and is in the range ofpH 5.0-pH10.0, more than 50% of the enzyme activity of the amylase Tcamy is maintained, and thereforethe amylase Tcamy has a good pH stability. Optimum temperature for the amylase Tcamy is 35DEG C, and at 0DEG C, 27% of activity of the amylase Tcamy is maintained. When heat treatment temperature is higher than the optimum temperature, the activity of the amylase Tcamy suddenly lowers. The amylase Tcamy is typical low temperature alpha-amylase.

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