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发明授权
CN108588099B 缺刻缘绿藻二酰甘油酰基转移酶(MiDGAT2C)基因序列及功能鉴定 失效 - 权利终止

缺刻缘绿藻二酰甘油酰基转移酶(MiDGAT2C)基因序列及功能鉴定
摘要：
本发明涉及缺刻缘绿藻二酰甘油酰基转移酶(MiDGAT2C)基因序列(如SEQ ID NO:7所示)及其应用。其特点表现在：MiDGAT2C不同于本课题组已克隆到的另外两个DGAT2基因。将该基因转化至酵母的TAG合成缺陷株H1246中表达，发现该基因的转入确实能使该酵母缺陷株H1246恢复合成TAG，证明了该基因编码蛋白具有合成TAG的功能。并发现MiDGAT2C对花生四烯酸‑CoA没有催化活性，但能将亚油酸‑、γ‑亚麻酸‑或α‑亚麻酸‑CoA的酰基转至DAG甘油骨架的sn‑3位以产生TAG，且能力相近。
摘要（英）：

The invention relates to a gene sequence (as shown in SEQ ID NO: 7) dimethylglycerol transferase (MiDGAT2C) in chlorophyta.and a application thereof, wherein the gene sequence dimethylglycerol transferase (MiDGAT2C) in chlorophyta is characterized in that: MiDGAT2C is different from the other two DGAT2 genes that the research group has cloned. The gene was transformed into the TAG-synthesized defective strain H1246 of the yeast, then it was found that the transfer of the gene did allow the yeast-defective strain H1246 to recover the synthetic TAG, and it was proved that the gene-encoding protein has the function of synthesizing the TAG. And it was found that the MiDGAT2C could not catalytically active a arachidonic acid-CoA, but can transfer an acid radical of a linoleic acid-, gamma linoleic acid, alpha-linolenic acid-CoA to the sn-3 bit of a DAG glycerin backbone to produce the TAG, the ability is similar.

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