The invention discloses migratory locust rhythm genes clk, cyc and per and the application thereof in control of insect diapauses. The migratory locust rhythm genes clk, cyc and per are cloned, specific double-stranded RNA interference sequence fragments dsRNA of the genes clk, cyc and per are developed, and the dsRNA is introduced into migratory locust to perform RNAi on the migratory locust rhythm genes clk, cyc and per by using an injection method. Experiments prove that the mRNA expression level of corresponding migratory locust genes is successfully interfered by utilizing the specific double-stranded RNA interference sequence fragments dsRNA of the rhythm genes clk, cyc and per, and the interference efficiency is obvious. The diapause rate of locust eggs produced by parent migratorylocusts is reduced after interference, which indicates that the rhythm genes clk, cyc and per achieve important effects in the insect diapauses induction process. The invention lays a foundation for developing a novel insecticide and reducing the population quantity of overwintering injurious insects.