The application provides a tissue and/or cell cryopreservation protective solution as well as preparation and application thereof. The tissue and/or cell cryopreservation protective solution comprisesa permeating protective agent and a non-permeating protective agent, wherein the cryopreservation protective solution further comprises platelet lysates. In addition, the application further providesa method for cryopreserving tissues and/or cells by using the tissue and/or cell cryopreservation protective solution provided by the application, as well as a cryopreserved tissue and/or cell and use of the cryopreserved tissue and/or cell.