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发明公开
CN108624549A 一种CHO DG44培养基及其应用 有权

一种CHO DG44培养基及其应用
摘要：
本发明属于生物技术领域，具体涉及一种CHO DG44培养基及其应用。所述培养基的成分中含有：OPM-CHO CD07、L-Glutamine、F-68、次黄嘌呤、胸腺嘧啶核苷、LONG® R3  IGF-I、亚油酸、吡哆胺二盐酸盐。本发明的CHO DG44培养基，用于培养CHO DG44细胞时，从细胞倍增时间，细胞最高密度和细胞培养时间上，都优于国外某知名品牌的CD DG44培养基，完全可以替代国外某知名品牌CD DG44培养基，更有利于CHO DG44细胞的培养。
摘要（英）：

The invention belongs to the technical field of biologics and particularly relates to a CHO DG44 (Chinese Hamster Ovary) culture medium and the application thereof. The culture medium comprises OPM-CHO CD 07, L-Glutamine, F-68, hypoxanthine, thymidine, LONG-R3 IGF-I, linoleic acid and pyridoxamine dihydrochloride. When the CHO DG44 culture medium provided by the invention is adopted to culture CHODG44 cells, the cell doubling time, the highest cell density and the cell culture time of the culture medium are all prior to those of a CHO DG44 culture medium of a foreign famous brand, the CHO DG44 culture medium of the foreign famous brand can be completely replaced by the culture medium, and culture of the CHO DG44 cells can be facilitated.

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