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发明公开
CN108624705A 一种假交替单胞菌快速PCR检测方法 有权

一种假交替单胞菌快速PCR检测方法
摘要：
本发明提供一种假交替单胞菌快速PCR检测方法，以假交替单胞菌基因为检测靶向基因，检测步骤包括引物合成、提取DNA、PCR反应、电泳检测和结果判定。此检测方法为国内首次公开的紫菜绿斑病病原检测方法，其检测准确率高，用假交替单胞菌人工感染条斑紫菜，以三对特异引物分别检测侵染紫菜，阳性检出率为100％。检测灵敏度高，采用本发明的三对引物均能特异性的检测假交替单胞菌，检测灵敏度分别为37CFU、3.7CFU、37CFU的细菌和2.37pg、23.7fg、2.37pg的DNA。
摘要（英）：

The invention provides a rapid PCR detection method for pseudoalteromonas nigrifaciens. The rapid PCR detection method uses a pseudoalteromonas nigrifaciens gene as a detection target gene, and comprises the detection steps of primer synthesis, DNA extraction, PCR reaction, electrophoresis detection and result determination. The rapid PCR detection method is the first method for detecting the pathogen of agar green spot disease in China, and the detection accuracy is high. Porphyra yezoensis is artificially infected with the pseudoalteromonas nigrifaciens, three pairs of specific primers are used for detecting the infected agar respectively, and the positive detection rate is 100%. The detection sensitivity is high, and the three pairs of primers can be used for specifically detecting thepseudoalteromonas nigrifaciens, and the detection sensitivity is 37 CFU, 3.7 CFU and 37 CFU of bacteria and 2.37pg, 23.7fg and 2.37 pg of DNA, respectively.

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