The invention relates to a steel structure positioning and conveying construction frame comprising a supporting bottom plate and a lifting device. The lifting device is mounted at the top of the supporting bottom plate and comprises a lifting adjustment mechanism, a limiting mechanism, a fixing bottom plate, a locking mechanism, side fixing branch chains and position clamping mechanisms; and the locking mechanism comprises a locking sliding plate, a locking pushing rod, a locking supporting frame, a locking hydraulic cylinder, a locking connecting plate, idler wheel frames, supporting idler wheels, locking clamping frames and clamping frame adjustment rods. According to the steel structure positioning and conveying construction frame, the problems that during conveying of existing I-shapedsteel, when the I-shaped steel is conveyed to the mounting position, the angle of the I-shaped steel cannot be adjusted, the I-shaped steel cannot be fixed in all directions during conveying of the I-shaped steel, security hidden dangers exist during conveying of the I-shaped steel, and the position of the I-shaped steel needs to be adjusted manually when the I-shaped steel is conveyed to the mounting position can be solved; and the functions that the I-shaped steel can be fixed in all directions and the I-shaped steel is conveyed to the proper position can be achieved.