The invention provides a device used for testing the puncture resistance of safety shoes and capable of simulating a real situation. The device comprises a testing frame, a press plate, a barbed nail,counterweight columns, a U-shaped clamping block, an adjustment threaded hole and an adjustment screw; a cavity is formed between a top plate and an insertion plate of the testing frame, a puncture hole is formed in the middle of the top of the testing frame, and hand wheel screws penetrate through through holes and are screwed into threaded connection holes in the two ends of the testing frame;the adjustment screw penetrates through a bearing on a top plate of a supporting plate and is screwed into the adjustment threaded hole in an adjustment column, a clamping frame with an open lower endis connected to the bottom of the adjustment column through bolts, a retractable rod on an electric cylinder penetrates through an end plate of the clamping frame and is welded with the U-shaped clamping block, and the counterweight columns can be screwed into counterweight threaded holes through threads of the front ends. In the process that a puncture testing press block drives the barbed nailto downwards puncture a testing part when freely falling, the situation of a real environment is simulated, the number of the counterweight columns is increased or decreased, and accordingly the puncture force of the barbed nail in contact with the testing part is changed.