The invention discloses a preparation method for medical anti-bacterial non-woven cloth. The method includes the steps that in an ice-water bath, borneol is added into tetrahydrofuran to be dissolved,pyridine is added, a tetrahydrofuran solution of chloroacetyl chloride is added for a reaction, and a material A is obtained through aftertreatment; sodium hydroxide is added into ethyl alcohol, beta-cyclodextrin is added, and a material B is obtained through stirring; the material B is added into ionic liquid, temperature is increased, the pH value is adjusted, the material A is added, and a material C is obtained after a reaction; the material C is added into water, an acetonitrile solution of paratoluensulfonyl chloride is added, and a material D is obtained after a reaction; the materialD is added into a sodium hydroxide solution to obtain conditioning fluid, and a material E is obtained after polyamide is added for treatment; the material E, viscose and spandex are added into a solvent to obtain a spinning solution; the spinning solution is subjected to static spinning, and then the medical anti-bacterial non-woven cloth is obtained. According to the preparation method for the medical anti-bacterial non-woven cloth, the process is simple, and the obtained medical anti-bacterial non-woven cloth is good in water absorption and bacterium resistance.