The invention discloses an evaluation and analysis method of harmful biological epidemic situations. According to the evaluation and analysis method of the epidemic situations provided by the invention, quantity needs to be investigated in a field, an investigation area is first determined in investigation; and then all red-imported-fire-ant nests in the GPS marker sample point area are investigated; all features, including ant nest sizes, presence of action of harmful ants, distances from the crowd and the like, of the red-imported-fire-ant nests are calculated and recorded; then an environment risk index I, an ant nest standard index Q and an ant nest sample ratio P are calculated on the basis of investigation according to the above evaluation method; and finally, an epidemic-situation index is calculated for grading, and proportions of the epidemic situations of all levels in sample points of the total investigation area are calculated. According to the method, influences of human action factors on the epidemic situations of the red imported fire ants in the area in an ecological environment are integrated, and an invasion situation of the red imported fire ants of the area canbe comprehensively and reasonably evaluated.