The invention provides a cultivation method of selenium-enriched tea. The cultivation method of the selenium-enriched tea comprises the following steps: 1) selecting land and preparing the selected land; 2) planting tea seedlings; 3) evenly spraying a mixed selenium solution onto branches of the tea trees 20 days before sprouting of tea leaves, wherein the concentration of the mixed selenium solution is 1 to 4000, and carrying out picking after sprouting of the tea leaves for a certain period of time. According to the cultivation method of the selenium-enriched tea, inorganic selenium is transformed into organic selenium under the action of inherent bio-transformation effects of the tea leaves during a growth process; at the same time, absorption and transformation effects during sproutingprocess of the tea leaves can be promoted by selenium in L-selenium-methylselenocysteine so as to ensure enrichment of selenium in a plurality of amino acids, proteins and the like of the tea leaves.Thus, types of nutrients including selenium-containing amino acids in the tea leaves are increased; and trace elements needed for tea leaf growth are also supplemented so as to improve absorption andtransformation effects of the tea leaves on inorganic selenium. As a result, tea leaves cultivated by adopting the cultivation method have higher organic selenium content compared with tea leaves singly containing inorganic selenium or organic selenium.