The invention discloses assembly equipment for a disposable oxygen gas humidifying bottle. The assembly equipment comprises a humidifying plug loading device, a transfer device, a ventilating tube loading device, a ventilating tube assembly device, a first assembly device, a second assembly device and a third assembly device, wherein the ventilating tube loading device comprises a material box, afirst conveyor belt, a loading block and an aligning assembly; the bottom of the material box is arranged in an inclined mode; a plurality of loading strips are arranged on the first conveyor belt; aloading slot is formed in the middle of the loading block; the loading slot is formed in the inclined mode; the aligning assembly comprises an aligning motor, an aligning block and four aligning strips; the cross section of the aligning block is of a rectangular structure, and four aligning slots which are distributed in the peripheral direction are formed in the aligning block; and the four aligning strips are fixedly connected with the aligning block. The assembly equipment has the beneficial effects that an assembly process for a humidifying plug, a ventilating tube, a connecting plug, a bottle body and a bottle lid can be automatically completed, production cost of products is reduced, and production speed of the disposable oxygen gas humidifying bottle is increased.