The invention discloses a Chinese herbal medicine environment-friendly disinfectant for livestock and poultry breeding and preparation and use methods thereof. The disinfectant is prepared from the following raw materials in parts by weight: 20-50 parts of radix isatidis, 30-50 parts of rhizoma coptidis, 30-50 parts of herba andrographis, 30-40 parts of radix scutellariae, 30-40 parts of fructus forsythiae, 20-40 parts of honeysuckle flowers, 10-20 parts of houttuynine sodium bisulfite, 10-20 parts of cortex phellodendri, 10-20 parts of fructus mume, 10-20 parts of herba leonuri, 10-20 parts of tree peony barks, 10-20 parts of spina gleditsiae, 5-10 parts of herba taraxaci, 5-10 parts of fructus chebulae and 5-10 parts of herba violae. The disinfectant is prepared through picking fifteen traditional Chinese medicines, i.e., the radix isatidis, the rhizoma coptidis, the herba andrographis, the radix scutellariae, the fructus forsythiae, the honeysuckle flowers, the houttuynine sodium bisulfite, the cortex phellodendri, the fructus mume, the herba leonuri, the tree peony barks, the spina gleditsiae, the herba taraxaci, the fructus chebulae and the herba violae, and each Chinese herbal medicine has a good antibacterial action. All the ingredients are reasonably combined and are complementary in effect, and thus, inhibiting and sterilizing effects are more outstanding.