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发明公开
CN108676898A 与猪产仔数相关的基因ANYZ的应用 无效 - 驳回

与猪产仔数相关的基因ANYZ的应用
摘要：
本发明涉及与猪产仔数相关的基因ANYZ的应用。ANYZ基因不同的基因型对于初产和经产的大白母猪效应不同，丹、加、美三种家系ANYZ基因B等位基因为优势基因，丹系初产及经产母猪AA型在活仔及总仔性能上均大于BB型；加系初产母猪AB型在活仔及总仔性能上均大于BB型；美系初产母猪AB型在活仔及总仔性能上均大于BB、AA型；美系和加系经产母猪BB型在活仔及总仔数高于AB型和AA型。本发明提供筛选种猪繁殖性能有效遗传标记的方法：包括：样品的采集与DNA的提取，目的基因ANYZ基因的PCR扩增，扩增产物琼脂糖凝胶电泳判型，最后进行数据处理分析，得出ANYZ基因对丹系、加系、美系三个群体活仔、总仔、健仔、死胎、木乃伊、初生重等因素的影响。
摘要（英）：

The invention relates to application of gene ANYZ related with pig litter size. Different genotypes of ANYZ gene have different effects on primiparous and multiparous large white sows. B alleles of Danmark, Canadian and American ANYZ genes are protogenes. AA type of Danmark primiparous and multiparous sows has greater living piglets and total piglet performance than BB type. AB type of Canadian primiparous sows has greater living piglets and total piglet performance than BB type. AB type of American primiparous sows has greater living piglets and total piglet performance than BB type and AA type. BB type of American and Canadian multiparous sows has greater living piglets and total litter size than AB type and AA type. The invention provides a method for screening valid genetic markers ofbreeding swine reproductive performance, comprising sample collection and DNA extraction, PCR amplification of the target gene ANYZ gene, agarose gel electrophoresis of amplification products, and data processing and analysis. Finally, the influence of the ANYZ gene on factors such as living piglets, total litter size, healthy piglets, fetal death, mummy, birth weight and the like of the Danmark,Canadian and American groups, is obtained.

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