The invention provides a plaster for treating pain as well as a preparation method and application thereof. The plaster is prepared from the following raw materials with medicine effects of radix angelicae pubescentis, asiatic toddalia root, cortex cinnamomi, rhizoma curcumae longae, rhizoma chuanxiong, frankincense, myrrh, semen sinapis and borneol; the auxiliary materials comprise rubber, lanolin, yellow vaseline, rosin, and zinc oxide. The plaster has the advantages that by compounding the radix angelicae pubescentis, the asiatic toddalia root, the cortex cinnamomi, the rhizoma curcumae longae, the rhizoma chuanxiong, the frankincense, the myrrh and the semen sinapis, the effects of dispelling wind and removing dampness, warming meridians and dispelling cold, activating blood circulation and removing blood stasis as well as dredging collaterals and relieving pain are realized; the health-care effects of relieving the uncomfortable symptom of local pain due to blockage of meridians and collaterals of joint pain, muscle ache, bruise injury and the like is relieved, and promoting rehabilitation are realized; the technology steps of the preparation method are simple and convenient;the dissolving of the rubber is not needed, so that the pollution to the environment and plaster by the use of solvent is avoided; the preparation method is more environmentally friendly, and the safety in use is high.