The invention discloses a preparation method of a meso-porous surface defect Cu-N-TiO2 microsphere photocatalytic material. The method comprises the following steps: 1) dissolving a surfactant in ethanol, and sequentially adding an ethanol solution of a copper-containing compound and deionized water while stirring, then adding a titanium-containing compound, continuously performing stirring for 30-50 s, standing the obtained solution to settle, and filtering the solution to obtain a precipitate; and 2) washing the precipitate with ethanol and/or water for 3 times or more, drying and grinding the washed precipitate to form a powder, hydrothermally reacting the powder, calcining a product obtained by the hydrothermal reaction in an air atmosphere, and carrying out high-temperature nitridingtreatment to obtain the meso-porous surface defect Cu-N-TiO2 microsphere photocatalytic material. The prepared microsphere photocatalytic material has uniform pores, good material dispersibility, large specific surface area and high photocatalytic efficiency.