The invention provides culture medium filling equipment. The culture medium filling equipment comprises an equipment body internally provided with a containing cavity. A filling tank is placed in thecontaining cavity. A liquid inlet pipeline is arranged at the bottom of the containing cavity. At least part of the liquid inlet pipeline extends in the height direction of the filling tank. A liquiddischarging pipeline is arranged on the side wall of the containing cavity. One opening end of the liquid inlet pipeline and one opening end of the liquid discharging pipeline stretch into the fillingtank. A pressurizing pump is further arranged in the equipment body and connected with the liquid inlet pipeline and the liquid discharging pipeline and is further connected with a water inlet pipeline. The water inlet pipeline is used for injecting water into the filling tank so that the pressurizing pump can be started. The pressurizing pump is used for driving culture media in the filling tankto circularly flow between the liquid discharging pipeline and the liquid inlet pipeline.