A method for simulating serial communication based on a querying mode is characterized in that serial port communication is simulated by means of a timer and a universal IO interface. The method comprises the following steps of in receiving data by an analog serial port, making the timer perform fixed-time sampling on level of the universal IO interface by first interruption time (T1); when a starting bit of a data message is queried, after sampling point delay preset time (T) of the first interruption time (T1), adjusting the first interruption time of the timer to second interruption time (T2) for sampling, performing in-position receiving of the data message; and after receiving of the stopping bit of the data message, restoring the timer for regularly quering the level of the universalIO interface by means of the first interruption time (T1).