The invention discloses a control system and method for warehouse ozone insecticidal sterilization. The system includes ozone concentration measuring equipment, a voltage/current converter and proportional-integral-derivative control equipment; the ozone concentration measuring equipment is installed in an exhaust outlet of a warehouse; the ozone concentration measuring equipment, the voltage/current converter and the proportional-integral-derivative control equipment are in successive connection; and the proportional-integral-derivative control equipment is separately connected to an ozone generator and an exhaust fan. According to the technical schemes, the ozone of the whole warehouse can be reached to a certain concentration, the best insecticidal sterilization effects can be achieved,secondary pollution can be avoided, and energy saving and environmental protection can be realized.