The invention belongs to the technical field of weed control, and discloses a control method of weeds in a rice field. Through agent treatment of soil, agent-spraying control of stems and leaves and manual weeding, the weeds in the rice field are controlled. In five years of investigation on the weeds in the rice field, 58 species of common weeds belonging to 24 families are found in the rice fields in the central region of Yunnan province, important weeds refer to panicum crus, cyperus difformis, echinochloa crusgalli and sagittaria sagittifolia, and main weeds refer to alisma orientalis, alternanthera philoxeroides, monochoria vaginalis, scirpus tabernaemontani and polygonum hydropiper. The first peak period of weed growth is from late March to early April, and the second peak period isfrom early June to early August. The weeds in the rice field can be controlled through agent treatment of soil, agent-spraying control of stems and leaves and manual weeding.