The invention discloses a preparation method of a laying hen feed. The preparation method comprises the following steps of (1) material pretreatment: separately crushing corns and soy meals, mixing the crushed corns and soy meals, and adding an obtained raw material A mixture in a Chinese medicinal material mixed solution for soaking to obtain a pretreated A material; (2) reactivation of a fermentation strain: mixing active dry yeast, glucoamylase, acidic protease, cellulose, xylanase, water and white sugar, and performing insulation and reactivation for 30min at 30-35 DEG C to obtain the fermentation strain; (3) fermentation: mixing the pretreated material A and the fermentation strain, performing fermentation for 36h at 40-45 DEG C, performing natural cooling to a room temperature, and performing drying to obtain a fermented material A; and (4) burdening: stirring and mixing the fermented material A, stone powder, an additive and vegetable oil. Meanwhile, the invention also disclosesapplication of the laying hen feed. By adopting the laying hen feed provided by the invention, the laying rate of the laying hen after being fed is high, and the feed-egg ratio is low.