The invention discloses a method for preparing a water-resistant and weather-resistant bamboo floor by using a biochemical modified soybean protein adhesive. The method includes the steps that dried carbonized bamboo is impregnated into an environment-friendly soybean protein adhesive, and after bamboo strip impregnation pretreatment is carried out, low temperature drying is performed; the impregnated bamboo strips are finely planed, glued and assembled, the assembled plate blanks are tied with ropes, are firstly pre-pressed, and then sent to a hot press, and the hot pressing temperature and pressure are set for hot pressing; and after the hot-pressed bamboo floor is cooled, sanding, end milling, and surface coating are performed, and the bamboo floor is obtained. After original bamboo issubjected to carbonized treatment, the degree of fiber damage is higher; impregnation pretreatment is performed on the carbonized bamboo strips by the environment-friendly soybean protein adhesive, sothat the weather-resistant performance of the bamboo floor is improved; and then the bamboo floor is prepared by the hot pressing process, hot pressing can enable bamboo sheets to be in contact withthe adhesive more closely, cell cavities in the bamboo cell structure are gradually and completely filled and densified, therefore the bonding ability between the bamboo sheets is improved, and the deformation resisting capability of the material is enhanced.