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发明公开
CN108753758A 超嗜热脂肪酶LipL及其相关生物材料与应用 失效 - 权利终止

超嗜热脂肪酶LipL及其相关生物材料与应用
摘要：
本发明公开了超嗜热脂肪酶LipL及其相关生物材料与应用。该超嗜热脂肪酶LipL为A1)‑A3)中的任一蛋白质：A1)氨基酸序列是SEQ ID No.2的蛋白质；A2)氨基酸序列是SEQ ID No.2的第53‑675位的蛋白质；A3)氨基酸序列是SEQ ID No.2的第54‑675位的蛋白质。该超嗜热脂肪酶的酶反应的最适温度为70℃，酶反应的最适pH为9.5，在37℃、pH值为9‑10条件下的脂肪酶酶活是在37℃、pH值为9.5条件下的脂肪酶酶活的80％以上，在70‑90℃、pH值为9.5条件下的脂肪酶酶活是在70℃、pH值为9.5条件下的脂肪酶酶活的97％以上。
摘要（英）：

The invention discloses a hyperthermophilic lipase LipL and related biomaterials and application thereof. The hyperthermophilic lipase LipL is one protein of A1)-A3): A1) protein with the amino acid sequence shown in SEQ ID No. 2; A2) 53rd to 675th sites of protein with the amino acid sequence shown in SEQ ID No. 2; and A3) 54th to 675th sites of protein with the amino acid sequence shown in SEQ ID No. 2. The optimum temperature of the enzyme reaction of the hyperthermophilic lipase is 70 DEG C, and the optimum pH of the enzyme reaction is 9.5. The lipase activity under the conditions that thetemperature is 37 DEG C, pH is 9-10 is 80% or more of the lipase activity under the conditions that the temperature is 37 DEG C, pH is 9.5. the lipase activity under the condition that the temperature is 70-90 DEG C, pH is 9.5 is 97% or moreof the lipase activity under the conditions that the temperature is 70 DEG C, pH is 9.5.

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