The invention discloses an ultra-depth-of-field image acquisition device. The ultra-depth-of-field image acquisition device comprises a base; a vertical column is installed on the base in the verticaldirection; an automatic lifting assembly is installed on the column in a sliding connection manner, and a bracket is installed on the automatic lifting assembly in the horizontal direction; a microscope eyepiece is mounted on one end of the bracket away from the vertical column; and a camera is mounted above the microscope eyepiece, and the camera is signally connected with a computer. The invention realizes accurate adjustment of the eyepiece displacement through a gearbox with the large transmission ratio, multi-level image acquisition of the object is realized; acquisition of the ultra-depth-of-field image is achieved, multi-stage gear transmission reduces the size of the device, and storage is facilitated; and a guiding wheel and a tensioning wheel can ensure stable transmission of aslider on a sliding rail, and image acquisition is more accurate.