The invention belongs to the field of plant culturing, and relates to a pepper whole-twig leaf picking method. The method comprises the steps that when the length of a lateral twig below a dichotomousbranch of the stem of a pepper plant reaches 1-6 cm, the lateral twig is smeared, and smearing is conducted for once every 2-8 days until there exists no germinated lateral twig below the dichotomousbranch of the stem; when a cotyledon starts becoming yellow, 2-5 pairs of true leaves above the cotyledon on the stem are removed; 2-5 pairs of blades of the true leaves from top to bottom are removed when a pair of true leaves left at the downmost position on the stem become yellow; all true leaves below the dichotomous branch of the stem are removed when a pair of true leaves left at the downmost position on the stem become yellow. The method has the advantages that the operation is convenient, a plant group structure can be improved to improve the ventilation and light transmission performance of the parts, close to the ground, of plants and reduce air humidity, the photosynthetic efficiency is improved, the plants can grow and develop to be more robust, plant diseases and insect pestscan be effectively controlled to increase the percentage of fertile fruit, lateral twigs and blades located below the dichotomous branch of the stem can be removed in batches, and therefore the influence on the growth of the plant is small.