The invention relates to a colloidal silver-containing calcium phosphate nano composite material as well as a preparation method and application thereof. The preparation method comprises the followingsteps: taking water-soluble calcium salt as a calcium source, water-soluble silver salt as a silver source and a phosphorus-containing biomolecules as a phosphorus source, and dissolving the water-soluble calcium salt, the water-soluble silver salt and the phosphorus-containing biomolecules into a solvent, thus obtaining a mixed solution, wherein the phosphorus-containing biomolecules are adenosine phosphate or/and adenosine phosphate salt; carrying out solvothermal reaction on the mixed solution, thus obtaining the colloidal silver-containing calcium phosphate nano composite material.