The invention provides a leave ginkgo biloba extract capsule. The invention further provides a preparation method and application of the leave ginkgo biloba extract capsule. According to the leave ginkgo biloba extract capsule and the preparation method thereof provided by the invention, the leave ginkgo biloba extract capsule with high quality can be obtained by optimized preparation steps and conditions. Compared with the leave ginkgo biloba extract capsule prepared with a conventional method, the leave ginkgo biloba extract capsule provided by the invention is stable in quality, low in failure rate, and good in consistency of leave ginkgo biloba extract capsules of different batches. In particular, the leave ginkgo biloba extract capsule is capable of effectively controlling ginkgolic acid and preventing the use of raw materials high in ginkgolic acid in the leave ginkgo biloba extract capsule, so that the safety of the leave ginkgo biloba extract capsule is effectively improved.