The invention discloses a portable multifunctional plant leaf hole puncher. The portable multifunctional plant leaf hole puncher is characterized in that six tool bits of different apertures and shapes are uniformly fixed to a tool bit turntable; a locking hole is corresponding formed under each tool bit; a locking stick is fixed to a pliers body through a screw behind the tool bit turntable; a bump at the front end of the locking stick is clamped into one of the locking holes to realize the fixing of the tool bit turntable and the tool bits. A corresponding tool bit gasket is selected from atool bit gasket accommodating groove in a hollow handle after the tool bits are fixed, and is clamped into a tool bit gasket groove to be fixed, and a centrifugal tube tray is fixed to the pliers bodythrough a sliding chute after a centrifugal tube is inserted into the centrifugal tube tray. A leaf is placed on the tool bit gasket horizontally, the hollow handle is connected with a spring, and ahinged shaft is connected with the locking stick, the tool bit gasket and the hollow handle. The portable multifunctional plant leaf hole puncher is simple in structure, and is convenient to use. Thehole punching sampling strength on leaves can be lowered effectively, the working efficiency is improved, real-time field sampling is realized, and the representativeness of an obtained sample and theaccuracy of an experimental research result are ensured more effectively.