The invention provides a nasal secretion acquisition retention device, an acquisition retention processing method, and applications of the nasal secretion acquisition retention device and the acquisition retention processing method, and belongs to the technical field of medical equipment and method. According to the nasal secretion acquisition retention device, the adopted elements are produced through standard production; operation is more convenient, safe, and healthy; errors caused by equipment and methods in artificial sample loading process are reduced; stabilized, standard nasal secretion samples with higher homogeneity can be obtained for clinical detection. The invention also provides the acquisition retention processing method of the nasal secretion. The nasal secretion acquisition retention device is used for acquisition and retention processing of nasal secretion; the nasal secretion liquid samples obtained through centrifugation are subjected to freeze condensation and freeze drying so as to obtain free-dried powder; the free-dried powder is diluted, and is subjected to corresponding detection, so that it is possible to ensure the dilution rate of nasal secretion is uniform, and a problem in the prior art that false negative detection results are caused by different dilution rate in nasal secretion acquisition process. The invention also provides applications of thenasal secretion acquisition retention device and the acquisition retention processing method.